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Research Update: Probiotic bacteria P. freudenreichii has impact on Salmonella

Divek Nair and Anup Kollanoor-Johny
Department of Animal Science, University of Minnesota

Recently, there has been increasing interest to use probiotic bacteria as an alternative to conventional antibiotics in controlling pathogens in animal agriculture, including poultry. Probiotics are live microorganisms that if administered in adequate quantity result in desired health benefits in the host, including reduction of pathogenic bacteria. Several probiotic bacteria, including Lactobacillus spp., Bacillus spp., Bifidobacterium, and Pediococcus have been used in the poultry industry, either individually or in combination. Propionibacterium freudenreichii, the focus of this study, has proven probiotic properties and is commonly present in fermented dairy products such as cheese.
We investigated the effects of P. freudenreichii, on Salmonella multiplication, motility, and association to an invasion of avian epithelial cells in vitro. Two sub-species of P. freudenreichii were tested against 3 Salmonella serotypes in poultry, namely, S. Enteritidis, S. Typhimurium, and S.Heidelberg, using co-culture-, motility, multiplication, cell association, and invasion assays.

Both strains of P. freudenreichii were effective in reducing or inhibiting multiplication of all 3 Salmonella serotypes in co-culture and turkey cecal contents. P. freudenreichii significantly reduced Salmonella motility. Cell culture studies revealed that P. freudenreichii associated with the avian epithelial cells effectively reduced S. Enteritidis, S. Heidelberg, and S. Typhimurium cell. Our current in vitro results indicate the potential of P. freudenreichii against Salmonella in poultry. Follow-up in vivo studies are underway to evaluate this possibility.

To view the full text: 2016 Poultry Science 00:1–11;

In vitro: studies performed with microorganisms, cells or biological molecules outside their normal biological context.

Serotype: a group of intimately related microorganisms distinguished by a common set of 

Antigens: any substance foreign to the body the evokes an immune response

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